Template Switching Oligo

Template Switching Oligo - The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during. Capillary electrophoresis (ce) was used to quantify primer elongation and template switching products, including concatemers that formed. Different approaches to perform 5′ race are available, and one particularly simple and powerful strategy is based on a. Template switching oligonucleotide and capturing oligo (dt) are used to. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. Reverse transcriptase then switches templates and continues replicating to the end of the oligo.

SMART technology overview
Schematic for cDNA synthesis by templateswitching. (Step 1) Primer
Fig. S12 Schematic representation of the tested oligodT / TSO
NanoCAGE A HighResolution Technique to Discover and Interrogate Cell
(A) First strand cDNA is initiated by priming with an oligo dT primer
(A) The TS mechanism is used for first strand cDNA synthesis. First, an
Template Switch Oligo
What Is A Template Switching Oligonucleotide
Model of cDNA synthesis that occurs in the absence (A) and presence (B
Template switching oligos (TS oligos, TSOs) for cDNA library

The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during. Reverse transcriptase then switches templates and continues replicating to the end of the oligo. Capillary electrophoresis (ce) was used to quantify primer elongation and template switching products, including concatemers that formed. Template switching oligonucleotide and capturing oligo (dt) are used to. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Different approaches to perform 5′ race are available, and one particularly simple and powerful strategy is based on a.

Template Switching Oligonucleotide And Capturing Oligo (Dt) Are Used To.

Use this set of oligonucleotides for template switching cdna synthesis and preamplification. Reverse transcriptase then switches templates and continues replicating to the end of the oligo. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end.

Different Approaches To Perform 5′ Race Are Available, And One Particularly Simple And Powerful Strategy Is Based On A.

Capillary electrophoresis (ce) was used to quantify primer elongation and template switching products, including concatemers that formed.

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